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This textbook has been conceptualized to provide a detailed description of the various aspects of Systems and Synthetic Biology, keeping the requirements of M.Sc. and Ph.D. students in mind. Also, it is hoped that this book will mentor young scientists who are willing to contribute to this area but do not know from where to begin.§The book has been divided into two sections.§The first section will deal with systems biology in terms of the foundational understanding, highlighting issues in biological complexity, methods of analysis and various aspects of modelling.§The second section deals with the engineering concepts, design strategies of the biological systems ranging from simple DNA/RNA fragments, switches and oscillators, molecular pathways to a complete synthetic cell will be described.§Finally, the book will offer expert opinions in legal, safety, security and social issues to present a well-balanced information both for students and scientists.