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Extending the range of enzymatic catalysis by using non-aqueous media considerably enlarges the range of enzyme applications and became a powerful approach in biochemistry and biotechnology. This book discusses several specific applications in nearly anhydrous media, aqueous-organic cosolvent mixtures and reverse micelles, including use of enzymes in fat and oil processing, peptide synthesis, sugar transformations and preparation of chiral compounds. A particularly promising approach of using reverse micelles for modifying proteins is also described. Almost each chapter covers background information, easy-to-follow protocols and illustrations of the specific applications.§Put together, the book is an important guideline for researchers and developers in biotechnological and pharmaceutical industries as well as academic sectors.