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In the present study, Streptomyces sp. MAPS 15, isolated from marine sponge was incorporated with commercial feed. The shrimps were orally fed with different doses of probiotic feed for a period of 14 days, and subjected to bacterial challenge experiments. It was found that shrimp fed with 108 CFUml 1 enhances the survival rate and immunity. To confirm the probiotic activity of MAPS 15 in natural conditions, efficacy validation was performed under wet lab and farm conditions (Hapa). The results indicate that, 108 CFUml 1 significantly improves survival rate, water quality and productivity of P. monodon. Therefore, this potential probionts could be utilized for the development of veterinary grade probiotic in aquaculture industries.