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Hemoglobin has been involved in the most significant advances in our understanding of modern genetics and molecular biology. Now, hemoblobin is again central to a new area: development of artificial blood (blood substitute.) This volume of "Methods in Enzymology" and its companion, Volume 232, will be indispensable to anyone with a serious interest in this emerging field. They completely update and extend the information presented in Volume 76 published a decade ago. It covers: preparation, purification, and analysis of human hemoglobin and of various non-human hemoglobins; preparation of hemoglobin hybrids and of model heme compounds; preparation of chemically modified hemoglobins and of recombinant hemoglobins; and analytical procedures presented for studying hemoglobin stability and degradation and for the formation of xenobiotic adducts of hemoglobin.