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The Gulf of Mexico is an open and dynamic marine ecosystem rich in natural resources but heavily impacted by human activities, including agricultural, industrial, commercial and coastal development. Nutrients and pollutants from coastal communities and dozens of rivers flow into the Gulf, including material from the Mississippi River watershed, which drains over one third of continental United States. The Gulf of Mexico has been continuously exposed to petroleum hydrocarbons for millions of years from natural oil and gas seeps on the sea floor, and more recently from oil drilling and production activities located in the water near and far from shore. Major accidental oil spills in the Gulf are infrequent. This is a two volume open access set under a CC BY-NC license.