Drugs of Abuse

A cutting-edge collection of updated and core techniques for the neurological study of drugs of abuse. Described in step-by-step detail by hands-on experts, these readily reproducible protocols cover a wide variety of coherent methods for gathering information on quantitative changes in protein and mRNA at both tissue and cellular levels. There are various methods for detecting single and multiple alterations in single and multiple gene expression, for analyzing the functional roles of genes and proteins, for studying the release kinetics of striatal dopamine, and for the quantitative measurement of such neurotransmitters as acetylcholine. Insightful review articles address key current topics on the effect of drugs of abuse on the brain.New molecular technologies have transformed our understanding of the neurological impact of drugs of abuse and opened many new possibilities for research. In Drugs of Abuse: Neurological Reviews and Protocols, leading scientists from all over the world review these novel research areas and provide a cutting-edge collection of updated and core techniques for the neurological study of drugs of abuse. Described in step-by-step detail, these readily reproducible protocols cover a wide variety of coherent methods for gathering information on quantitative changes in protein and mRNA at both tissue and cellular levels. There are various methods for detecting single and multiple alterations in single and multiple gene expression, for analyzing the functional roles of genes and proteins, for studying the release kinetics of striatal dopamine, and for the quantitative measurement of such neurotransmitters as acetylcholine. Additional methods are provided for primary neural cell culture preparation, for an immunohistochemical method to examine cellular proliferation and differentiation, and for a culture method to grow viable neural progenitors. All contributors have considerable experience with the protocols covered, and each protocol has been thoroughly tried and successfully tested in the laboratory. Helpful notes for each method discuss tricks-of-the-trade, pitfalls to avoid, and troubleshooting. §State-of-the-art and highly practical, Drugs of Abuse: Neurological Reviews and Protocols offers insightful reviews of key current topics and a core collection of readily reproducible techniques essential for any neurobiological laboratory carrying out either basic analyses or seeking novel research approaches.